Heat Resistance of Bacillus Spores at Various Relative Humidities
نویسندگان
چکیده
منابع مشابه
The heat resistance of bacterial spores at various water activities.
The heat resistance of the spores of six species of bacteria varied with water activity (a,) at which the spores were heated, although the magnitude of the changes differed greatly between species. At all a, values there was an approximately linear relation between the logarithm of the number of viable spores and the time of heating. The slopes of these straight lines were used to describe the ...
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Heat shock of dormant spores of Bacillus stearothermophilus ATCC 7953 at 100 or 80 degrees C for short times, the so-called activation or breaking of dormancy, was investigated by separating the resulting spores by buoyant density centrifugation into a band at 1.240 g/ml that was distinct from another band at 1.340 g/ml, the same density as the original spores. The proportion of spores at 1.240...
متن کاملHeat Resistance and Population Stability of Lyophilized Bacillus subtilis Spores.
Bacillus subtilis 5230 spores were lyophilized in 0.067 M phosphate buffer and stored at 2 to 8 degrees C for 9 to 27 months. The lyophilized spores were reconstituted with buffer or 0.9% saline, and the heat resistance was determined in a thermoresistometer. Lyophilization had no effect on the heat resistance of the spores but did result in a slight decrease in population (</=0.3-logarithm red...
متن کاملSuperdormant spores of Bacillus species have elevated wet-heat resistance and temperature requirements for heat activation.
Purified superdormant spores of Bacillus cereus, B. megaterium, and B. subtilis isolated after optimal heat activation of dormant spores and subsequent germination with inosine, d-glucose, or l-valine, respectively, germinate very poorly with the original germinants used to remove dormant spores from spore populations, thus allowing isolation of the superdormant spores, and even with alternate ...
متن کاملHeat shock proteins do not influence wet heat resistance of Bacillus subtilis spores.
Spores of Bacillus subtilis are significantly more resistant to wet heat than are their vegetative cell counterparts. Analysis of the effects of mutations in and the expression of fusions of a coding gene for a thermostable beta-galactosidase to a number of heat shock genes has shown that heat shock proteins play no significant role in the wet heat resistance of B. subtilis spores.
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1981
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.42.4.692-697.1981